Policosanol Stimulates Osteoblast Differentiation via Adenosine Monophosphate-Activated Protein Kinase-Mediated Expression of Insulin-Induced Genes 1 and 2

Policosanol is known as a hypocholesterolemic compound and derived from plants such sugar cane corn. can lower blood pressure or inhibit adipogenesis, but its effect on osteogenic differentiation the molecular mechanism unclear. This study aims to investigate of policosanol in MC3T3-E1 cells zebrafish models. Administration into induced expression genes distal-less homeobox 5 (Dlx5) runt-related transcription factor 2 (Runx2). Alkaline phosphatase activity extracellular mineralization also increased. promoted activation adenosine monophosphate-activated protein kinase (AMPK) insulin-induced (INSIGs) regulation INSIGs modulated osteoblast differentiation. AMPK through transfection constitutively active form (CA-AMPK) increased expression, whereas policosanol-induced was suppressed by inhibitor (Com. C). Furthermore, effects were verified zebrafish. Amputated caudal fin rays regenerated treatment. Taken together, these results show that increases contributes regeneration via AMPK-mediated suggesting has potential an agent.